RT Journal Article
T1 Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells.
A1 Sanchez-Gomar, Ismael
A1 Benitez-Camacho, Josefa
A1 Cejudo-Bastante, Cristina
A1 Casas, Lourdes
A1 Moreno-Luna, Rafael
A1 Mantell, Casimiro
A1 Duran-Ruiz, Mª Carmen
K1 ECFCs
K1 Angiogenesis
K1 Antioxidants
K1 Cell therapy
K1 Grape pomace
K1 Inflammation
K1 Mango leaves
K1 Olive leaves
K1 Polyphenols
K1 Proliferation
AB Cardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularization; however, under the oxidative environment that prevails in ischemic areas, these cells become impaired. Thus, it is necessary to investigate strategies to enhance their regenerative properties. Antioxidant substances, such as polyphenols, have been shown to be useful for this purpose. In the current study we evaluated the potential of mango leaves, olive leaves and red grape pomace extracts, rich in polyphenols, to promote ECFC reparative effects. For this, aqueous and ethanolic extracts of the aforementioned raw materials were obtained by pressurized liquid extraction (PLE). After evaluating the polyphenol content and the antioxidant activity, in vitro assays were carried out, and we found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells. Overall, mango leaves ethanolic extract provided the most promising results, but all three extracts ameliorated the functionality of ECFCs.
PB MDPI
SN 2076-3921
YR 2022
FD 2022-04-24
LK http://hdl.handle.net/10668/20780
UL http://hdl.handle.net/10668/20780
LA en
DS RISalud
RD Apr 19, 2025