RT Journal Article
T1 Blood Rheological Characterization of beta-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry
A1 Mendez-Mora, Lourdes
A1 Cabello-Fusares, Maria
A1 Ferre-Torres, Josep
A1 Riera-Llobet, Carla
A1 Krishnevskaya, Elena
A1 Trejo-Soto, Claudia
A1 Payan-Pernia, Salvador
A1 Hernandez-Rodriguez, Ines
A1 Morales-Indiano, Cristian
A1 Alarcon, Tomas
A1 Vives-Corrons, Joan-Lluis
A1 Hernandez-Machado, Aurora
K1 beta-thalassemia trait
K1 iron deficiency (anemia)
K1 anemia
K1 hemorheology
K1 rheology
K1 microfluidics
K1 blood rheology
K1 microrheometer
K1 Cell membrane
K1 Deformation
K1 Aggregation
K1 Viscosity
AB The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (beta-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of beta-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, beta-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA).
PB Frontiers Research Foundation
YR 2021
FD 2021-10-21
LK https://hdl.handle.net/10668/27834
UL https://hdl.handle.net/10668/27834
LA en
NO Méndez-Mora L, Cabello-Fusarés M, Ferré-Torres J, Riera-Llobet C, Krishnevskaya E, Trejo-Soto C, et al. Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry. Front Physiol. 2021 Oct 21;12:761411.
DS RISalud
RD Apr 12, 2025