RT Journal Article
T1 Measurement of Serum Testosterone in Nondiabetic Young Obese Men: Comparison of Direct Immunoassay to Liquid Chromatography-Tandem Mass Spectrometry.
A1 Martínez-Escribano, Ana
A1 Maroto-García, Julia
A1 Ruiz-Galdón, Maximiliano
A1 Barrios-Rodríguez, Rocío
A1 Álvarez-Millán, Juan J
A1 Cabezas-Sánchez, Pablo
A1 Plaza-Andrades, Isaac
A1 Molina-Vega, María
A1 Tinahones, Francisco J
A1 Queipo-Ortuño, María Isabel
A1 Fernández-García, José Carlos
K1 chromatography
K1 immunoassay
K1 obesity
K1 testosterone
AB Hypoandrogenemia, a frequent finding in men with obesity, is defined by low concentrations of serum testosterone. Although immunoassay (IA) is the most used method for the determination of this steroid in clinical practice, liquid chromatography-mass spectrometry (LC-MS/MS) is considered a more reliable method. In this study, we aimed to compare IA versus LC-MS/MS measurement for the diagnosis of hypoandrogenemia in a cohort of 273 nondiabetic young obese men. Mean total testosterone (TT) levels were 3.20 ± 1.24 ng/mL for IA and 3.78 ± 1.4 ng/mL for LC-MS/MS. 53.7% and 26.3% of patients were classified as presenting hypoandrogenemia with IA and LC-MS/MS, respectively. Considering LC-MS/MS as the reference method, sensitivity and specificity of IA were 91.4% (95% CI 82.3-96.8) and 61.1% (95% CI 54.0-67.8), respectively. IA presented an AUC of 0.879 (95% CI 0.83-0.928). Multivariate regression analysis indicated that sex hormone-binding globulin (SHBG) concentrations (p = 0.002) and insulin resistance (p = 0.008) were factors associated with discrepant IA values. In conclusion, the determination of TT by IA in nondiabetic young men with obesity yields lower concentrations of TT than LC-MS/MS, resulting in an equivocal increased diagnosis of hypoandrogenemia, which could lead to inaccurate diagnosis and unnecessary treatment.
YR 2020
FD 2020-12-19
LK http://hdl.handle.net/10668/16834
UL http://hdl.handle.net/10668/16834
LA en
DS RISalud
RD Apr 10, 2025