RT Journal Article
T1 Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples.
A1 Martínez-Huélamo, Miriam
A1 Tulipani, Sara
A1 Jáuregui, Olga
A1 Valderas-Martínez, Palmira
A1 Vallverdú-Queralt, Anna
A1 Estruch, Ramón
A1 Torrado, Xavier
A1 Lamuela-Raventós, Rosa M
K1 Polyphenol metabolites
K1 Validation
K1 Tomato
K1 Sauce
K1 Microbiota
K1 Metabolites
K1 Biosamples
K1 Blood
K1 Urine
K1 Fluidos corporales
K1 Cromatografía líquida de alta presión
K1 Voluntarios sanos
K1 Límite de detección
K1 Fenoles
K1 Humanos
K1 Espectrometría de masas en tándem
AB An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame.
PB MDPI
YR 2015
FD 2015-11-16
LK http://hdl.handle.net/10668/2361
UL http://hdl.handle.net/10668/2361
LA en
NO Martínez-Huélamo M, Tulipani S, Jáuregui O, Valderas-Martínez P, Vallverdú-Queralt A, Estruch R, et al. Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples. Molecules. 2015; 20(11):20409-25
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 8, 2025