RT Journal Article
T1 ADAR-mediated RNA editing of DNA:RNA hybrids is required for DNA double strand break repair.
A1 Jimeno, Sonia
A1 Prados-Carvajal, Rosario
A1 Fernández-Ávila, María Jesús
A1 Silva, Sonia
A1 Silvestris, Domenico Alessandro
A1 Endara-Coll, Martín
A1 Rodríguez-Real, Guillermo
A1 Domingo-Prim, Judit
A1 Mejías-Navarro, Fernando
A1 Romero-Franco, Amador
A1 Jimeno-González, Silvia
A1 Barroso, Sonia
A1 Cesarini, Valeriana
A1 Aguilera, Andrés
A1 Gallo, Angela
A1 Visa, Neus
A1 Huertas, Pablo
AB The maintenance of genomic stability requires the coordination of multiple cellular tasks upon the appearance of DNA lesions. RNA editing, the post-transcriptional sequence alteration of RNA, has a profound effect on cell homeostasis, but its implication in the response to DNA damage was not previously explored. Here we show that, in response to DNA breaks, an overall change of the Adenosine-to-Inosine RNA editing is observed, a phenomenon we call the RNA Editing DAmage Response (REDAR). REDAR relies on the checkpoint kinase ATR and the recombination factor CtIP. Moreover, depletion of the RNA editing enzyme ADAR2 renders cells hypersensitive to genotoxic agents, increases genomic instability and hampers homologous recombination by impairing DNA resection. Such a role of ADAR2 in DNA repair goes beyond the recoding of specific transcripts, but depends on ADAR2 editing DNA:RNA hybrids to ease their dissolution.
YR 2021
FD 2021-09-17
LK http://hdl.handle.net/10668/18523
UL http://hdl.handle.net/10668/18523
LA en
DS RISalud
RD Apr 17, 2025