RT Journal Article
T1 PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia.
A1 Moral, Raimundo M G del
A1 Gómez-Morales, Mercedes
A1 Hernández-Cortés, Pedro
A1 Aguilar, David
A1 Caballero, Trinidad
A1 Aneiros-Fernández, Jose
A1 Caba-Molina, Mercedes
A1 Rodríguez-Martínez, M Dolores
A1 Peralta, Andreina
A1 Galindo-Moreno, Pablo
A1 Osuna, Antonio
A1 Oliver, F Javier
A1 Moral, Raimundo G del
A1 O'Valle, Francisco
K1 poly(ADP-ribose)polymerase-1, mouse
K1 3,4-dihydro-5-(4-(1-piperidinyl)butoxy)-1(2H)-isoquinolinone
K1 Frio
K1 Isquemia fría
K1 Riñón
K1 Necrosis tubular aguda
K1 Ratones
K1 Ratones consanguíneos C57BL
K1 Ratones noqueados
K1 Microscopía electrónica
K1 Piperidinas
K1 Poli(ADP-Ribosa) polimerasas
K1 Daño por reperfusión
K1 Animales
AB We test the hypothesis that PARP inhibition can decrease acute tubular necrosis (ATN) and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of Wisconsin (UW) solution. Material and Methods. We used 30 male Parp1(+/+) wild-type and 15 male Parp1(0/0) knockout C57BL/6 mice. Fifteen of these wild-type mice were pretreated with 3,4-dihydro-5-[4-(1-piperidinyl)butoxyl]-1(2H)-isoquinolinone (DPQ) at a concentration of 15 mg/kg body weight, used as PARP inhibitor. Subgroups of mice were established (A: IR 45 min/6 h; B: IR + 48 h in UW solution; and C: IR + 48 h in UW solution plus DPQ). We processed samples for morphological, immunohistochemical, ultrastructural, and western-blotting studies. Results. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with PARP inhibitor DPQ plus DPQ supplementation in UW solution decreased PARP-1 nuclear expression in renal tubules and renal damage. Parp1(0/0) knockout mice were more resistant to IR-induced renal lesion. In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored organs at transplantation.
PB Hindawi Publishing Corporation
YR 2013
FD 2013
LK http://hdl.handle.net/10668/1810
UL http://hdl.handle.net/10668/1810
LA en
NO Moral RMG del, Gómez-Morales M, Hernández-Cortés P, Aguilar D, Caballero T, Aneiros-Fernández J, et al. PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia. ScientificWorldJournal; 2013:486574
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 8, 2025