RT Journal Article
T1 Proteomic Profile Associated With Loss of Spontaneous Human Immunodeficiency Virus Type 1 Elite Control.
A1 Rodríguez-Gallego, Esther
A1 Tarancón-Diez, Laura
A1 García, Felipe
A1 Del Romero, Jorge
A1 Benito, Jose Miguel
A1 Alba, Verónica
A1 Herrero, Pol
A1 Rull, Anna
A1 Dominguez-Molina, Beatriz
A1 Martinez-Madrid, Onofre
A1 Martin-Pena, Luisa
A1 Pulido, Federico
A1 León, Agathe
A1 Rodríguez, Carmen
A1 Rallón, Norma
A1 Peraire, Joaquim
A1 Viladés, Consuelo
A1 Leal, Manuel
A1 Vidal, Francesc
A1 Ruiz-Mateos, Ezequiel
A1 HIV-1 Elite Controllers Study Group (ECRIS), Spanish AIDS Research Network
K1 HIV-1
K1 biomarkers
K1 elite controllers
K1 loss of control
K1 proteomic profile
AB Elite controllers (ECs) spontaneously control plasma human immunodeficiency virus type 1 (HIV-1) RNA without antiretroviral therapy. However, 25% lose virological control over time. The aim of this work was to study the proteomic profile that preceded this loss of virological control to identify potential biomarkers. Plasma samples from ECs who spontaneously lost virological control (transient controllers [TCs]), at 2 years and 1 year before the loss of control, were compared with a control group of ECs who persistently maintained virological control during the same follow-up period (persistent controllers [PCs]). Comparative plasma shotgun proteomics was performed with tandem mass tag (TMT) isobaric tag labeling and nanoflow liquid chromatography coupled to Orbitrap mass spectrometry. Eighteen proteins exhibited differences comparing PC and preloss TC timepoints. These proteins were involved in proinflammatory mechanisms, and some of them play a role in HIV-1 replication and pathogenesis and interact with structural viral proteins. Coagulation factor XI, α-1-antichymotrypsin, ficolin-2, 14-3-3 protein, and galectin-3-binding protein were considered potential biomarkers. The proteomic signature associated with the spontaneous loss of virological control was characterized by higher levels of inflammation, transendothelial migration, and coagulation. Galectin-3 binding protein could be considered as potential biomarker for the prediction of virological progression and as therapeutic target in ECs.
YR 2019
FD 2019
LK http://hdl.handle.net/10668/13070
UL http://hdl.handle.net/10668/13070
LA en
DS RISalud
RD Apr 17, 2025