RT Journal Article
T1 Generation of a humanized Aβ expressing mouse demonstrating aspects of Alzheimer's disease-like pathology
A1 Baglietto-Vargas, David
A1 Forner, Stefania
A1 Cai, Lena
A1 Martini, Alessandra C.
A1 Trujillo-Estrada, Laura
A1 Swarup, Vivek
A1 Nguyen, Marie Minh Thu
A1 Do Huynh, Kelly
A1 Javonillo, Dominic I.
A1 Tran, Kristine Minh
A1 Phan, Jimmy
A1 Jiang, Shan
A1 Kramár, Enikö A.
A1 Nuñez-Diaz, Cristina
A1 Balderrama-Gutierrez, Gabriela
A1 Garcia, Franklin
A1 Childs, Jessica
A1 Rodriguez-Ortiz, Carlos J.
A1 Garcia-Leon, Juan Antonio
A1 Kitazawa, Masashi
A1 Shahnawaz, Mohammad
A1 Matheos, Dina P.
A1 Ma, Xinyi
A1 Da Cunha, Celia
A1 Walls, Ken C.
A1 Ager, Rahasson R.
A1 Soto, Claudio
A1 Gutierrez, Antonia
A1 Moreno-Gonzalez, Ines
A1 Mortazavi, Ali
A1 Tenner, Andrea J.
A1 MacGregor, Grant R.
A1 Wood, Marcelo
A1 Green, Kim N.
A1 LaFerla, Frank M.
K1 Periodic acid-schiff
K1 Exon
K1 Cognition
K1 Mutation
K1 Gene expression
K1 Alzheimer disease
K1 Reacción del ácido peryódico de Schiff
K1 Exones
K1 Cognición
K1 Mutación
K1 Expresión génica
K1 Enfermedad de Alzheimer
AB The majority of Alzheimer's disease (AD) cases are late-onset and occur sporadically, however most mouse models of the disease harbor pathogenic mutations, rendering them better representations of familial autosomal-dominant forms of the disease. Here, we generated knock-in mice that express wildtype human Aβ under control of the mouse App locus. Remarkably, changing 3 amino acids in the mouse Aβ sequence to its wild-type human counterpart leads to age-dependent impairments in cognition and synaptic plasticity, brain volumetric changes, inflammatory alterations, the appearance of Periodic Acid-Schiff (PAS) granules and changes in gene expression. In addition, when exon 14 encoding the Aβ sequence was flanked by loxP sites we show that Cre-mediated excision of exon 14 ablates hAβ expression, rescues cognition and reduces the formation of PAS granules.
PB Springer Nature
YR 2021
FD 2021-04-23
LK http://hdl.handle.net/10668/4585
UL http://hdl.handle.net/10668/4585
LA en
NO Baglietto-Vargas D, Forner S, Cai L, Martini AC, Trujillo-Estrada L, Swarup V, et al. Generation of a humanized Aβ expressing mouse demonstrating aspects of Alzheimer's disease-like pathology. Nat Commun. 2021 Apr 23;12(1):2421
DS RISalud
RD Apr 18, 2025