RT Journal Article
T1 Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests
A1 Molina, Noemi
A1 Martin-Serrano, Angela
A1 Fernandez, Tahia D.
A1 Tesfaye, Amene
A1 Najera, Francisco
A1 Torres, María J.
A1 Mayorga, Cristobalina
A1 Vida, Yolanda
A1 Montañez, Maria I.
A1 Perez-Inestrosa, Ezequiel
K1 PAMAM
K1 Dendrimeric antigens
K1 Penicillin
K1 Drug allergy
K1 Basophil activation tests
K1 Penicilinas
K1 Hipersensibilidad a las drogas
K1 Prueba de desgranulación de los Basófilos
AB Dendrimeric Antigens (DeAns) consist of dendrimers decorated with multiple units of drug antigenic determinants. These conjugates have been shown to be a powerful tool for diagnosing penicillin allergy using in vitro immunoassays, in which they are recognized by specific IgE from allergic patients. Here we propose a new diagnostic approach using DeAns in cellular tests, in which recognition occurs through IgE bound to the basophil surface. Both IgE molecular recognition and subsequent cell activation may be influenced by the tridimensional architecture and size of the immunogens. Structural features of benzylpenicilloyl-DeAn and amoxicilloyl-DeAn (G2 and G4 PAMAM) were studied by diffusion Nuclear Magnetic Resonance (NMR) experiments and are discussed in relation to molecular dynamics simulation (MDS) observations. IgE recognition was clinically evaluated using the basophil activation test (BAT) for allergic patients and tolerant subjects. Diffusion NMR experiments, MDS and cellular studies provide evidence that the size of the DeAn, its antigen composition and tridimensional distribution play key roles in IgE-antigen recognition at the effector cell surface. These results indicate that the fourth generation DeAns induce a higher level of basophil activation in allergic patients. This approach can be considered as a potential complementary diagnostic method for evaluating penicillin allergy.
PB MDPI
YR 2018
FD 2018-04-24
LK http://hdl.handle.net/10668/3344
UL http://hdl.handle.net/10668/3344
LA en
NO Molina N, Martin-Serrano A, Fernandez TD, Tesfaye A, Najera F, Torres MJ, et al. Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests. Molecules. 2018 Apr 24;23(5):997.
DS RISalud
RD Apr 5, 2025