RT Journal Article
T1 In Vitro Digestion of Human Milk: Influence of the Lactation Stage on the Micellar Carotenoids Content.
A1 Xavier, Ana A O
A1 Garrido-López, Juan E
A1 Aguayo-Maldonado, Josefa
A1 Garrido-Fernández, Juan
A1 Fontecha, Javier
A1 Pérez-Gálvez, And Antonio
K1 breastfeeding
K1 carotenes and xanthophylls
K1 human colostrum
K1 in vitro digestibility
K1 micellar lipids
K1 newborn
AB Human milk is a complex fluid with nutritive and non-nutritive functions specifically structured to cover the needs of the newborn. The present study started with the study of carotenoid composition during progress of lactation (colostrum, collected at 3-5 d postpartum; mature milk, collected at 30 d postpartum) with samples donated from full-term lactating mothers (women with no chronic diseases, nonsmokers on a regular diet without supplements, n = 30). Subsequently, we applied an in vitro protocol to determine the micellarization efficiency of the carotenoids, which were separated by HPLC and quantified by the external standard method. That in vitro protocol is tailored for the biochemistry of the digestive tract of a newborn. To the best of our knowledge, the present study is the first report of carotenoids micellar contents, obtained in vitro. This study reveals, from the in vitro perspective, that colostrum and mature milk produce significant micellar contents of carotenoids despite lipids in milk are within highly complex structures. Indeed, the lactation period develops some influence on the micellarization efficiency, influence that might be attributed to the dynamics of the milk fat globule membrane (MFGM) during the progress of lactation.
SN 2076-3921
YR 2019
FD 2019-08-07
LK https://hdl.handle.net/10668/26793
UL https://hdl.handle.net/10668/26793
LA en
DS RISalud
RD Apr 10, 2025