RT Journal Article
T1 Direct regulation of fibroblast growth factor 23 by energy intake through mTOR
A1 Vidal, Angela
A1 Rios, Rafael
A1 Pineda, Carmen
A1 Lopez, Ignacio
A1 Muñoz-Castañeda, Juan R.
A1 Rodriguez, Mariano
A1 Aguilera-Tejero, Escolastico
A1 Raya, Ana I.
K1 Cells
K1 Rats
K1 Energy intake
K1 RNA, messenger
K1 Fibroblast growth factor-23
K1 Glucose
K1 TOR serine-threonine kinases
K1 Células
K1 Ratas
K1 Ingestión de energía
K1 ARN mensajero
K1 Factores de crecimiento de fibroblastos 23
K1 Glucosa
K1 Serina-treonina quinasas TOR
AB To test the hypothesis that fibroblast growth factor 23 (FGF23) is directly regulated by energy intake, in vivo and in vitro experiments were conducted. Three groups of rats were fed diets with high (HC), normal (NC) and low (LC) caloric content that resulted in different energy intake. In vitro, UMR106 cells were incubated in high (HG, 4.5 g/l) or low glucose (LG, 1 g/l) medium. Additional treatments included phosphorus (P), mannitol, rapamycin and everolimus. Intestinal absorption of P and plasma P concentrations were similar in the three groups of rats. As compared with NC, plasma FGF23 concentrations were increased in HC and decreased in the LC group. A significant correlation between energy intake and plasma FGF23 concentrations was observed. In vitro, mRNA FGF23 was significantly higher in UMR106 cells cultured in HG than in LG. When exposed to high P, mRNA FGF23 increased but only when cells were cultured in HG. Cells incubated with HG and mechanistic target of rapamycin (mTOR) inhibitors expressed low mRNA FGF23, similar to the values obtained in LG. In conclusion, this study shows a direct regulation of FGF23 production by energy availability and demonstrates that the mTOR signaling pathway plays a central role in this regulatory system.
PB Springer Nature
YR 2020
FD 2020-02-04
LK http://hdl.handle.net/10668/3556
UL http://hdl.handle.net/10668/3556
LA en
NO Vidal A, Rios R, Pineda C, Lopez I, Muñoz-Castañeda JR, Rodriguez M, et al. Direct regulation of fibroblast growth factor 23 by energy intake through mTOR. Sci Rep. 2020 Feb 4;10(1):1795
DS RISalud
RD Apr 7, 2025