RT Journal Article
T1 Differential iNKT and T Cells Activation in Non-Alcoholic Fatty Liver Disease and Drug-Induced Liver Injury.
A1 Caballano-Infantes, Estefanía
A1 Garcia-Garcia, Alberto
A1 Lopez-Gomez, Carlos
A1 Cueto, Alejandro
A1 Robles-Diaz, Mercedes
A1 Ortega-Alonso, Aida
A1 Martin-Reyes, Flores
A1 Alvarez-Alvarez, Ismael
A1 Arranz-Salas, Isabel
A1 Ruiz-Cabello, Francisco
A1 Lucena, Isabel M
A1 Garcia-Fuentes, Eduardo
A1 Andrade, Raul J
A1 Garcia-Cortes, Miren
K1 drug-induced liver injury (DILI)
K1 immune response
K1 immunophenotype
K1 liver fibrosis
K1 non-alcoholic fatty liver disease (NAFLD)
AB Non-alcoholic fatty liver disease (NAFLD) and idiosyncratic drug-induced liver injury (DILI) could share molecular mechanisms involving the immune system. We aimed to identify activation immunological biomarkers in invariant natural killer T (iNKT) and CD4/CD8+ T cells in NAFLD and DILI. We analyzed the activation profile (CD69, CD25, and HLA-DR) and natural killer group 2 member D (NKG2D) on iNKT cells, and CD4/CD8 T cells in peripheral blood mononuclear cells from NAFLD, with or without significant liver fibrosis, and DILI patients. There was an increase in iNKT cells in NAFLD patients compared to DILI or control subjects. Regarding the cellular activation profile, NAFLD with significant liver fibrosis (F ≥ 2) displayed higher levels of CD69+iNKT cells compared to NAFLD with none or mild liver fibrosis (F ≤ 1) and control patients. CD69+iNKT positively correlated with insulin resistance, aspartate aminotransferase (AST) level, liver fibrosis-4 index (FIB4) and AST to Platelet Ratio Index (APRI). DILI patients showed an increase in CD69+ and HLA-DR+ in both CD4+ and CD8+ T cells, detecting the most relevant difference in the case of CD69+CD8+ T cells. CD69+iNKT may be a biomarker to assess liver fibrosis progression in NAFLD. CD69+CD8+ T cells were identified as a potential distinctive biomarker for distinguishing DILI from NAFLD.
PB MDPI AG
SN 2227-9059
YR 2021
FD 2021-12-23
LK http://hdl.handle.net/10668/20811
UL http://hdl.handle.net/10668/20811
LA en
NO Caballano-Infantes E, García-García A, Lopez-Gomez C, Cueto A, Robles-Diaz M, Ortega-Alonso A et al. Differential iNKT and T Cells Activation in Non-Alcoholic Fatty Liver Disease and Drug-Induced Liver Injury. Biomedicines. 2021 Dec 28;10(1):55
NO This work was supported in part by a grant from the Instituto de Salud Carlos III (Spain) (PI18/01804, PI19/00883, PI21/01248), from the Consejería de Economía, Conocimiento, Empresas y Universidad (Junta de Andalucía, Spain) (UMA18-FEDERJA-194, PI18-RT-3364), and from the Consejería de Salud (Junta de Andalucía, Spain) (PI-0285-2016). This study has been co-funded by FEDER funds (“A way to make Europe”) (“Andalucía se mueve con Europa”).
DS RISalud
RD Apr 7, 2025