RT Journal Article
T1 Effects on Steroid 5-Alpha Reductase Gene Expression of Thai Rice Bran Extracts and Molecular Dynamics Study on SRD5A2.
A1 Khantham, Chiranan
A1 Yooin, Wipawadee
A1 Sringarm, Korawan
A1 Sommano, Sarana Rose
A1 Jiranusornkul, Supat
A1 Carmona, Francisco David
A1 Nimlamool, Wutigri
A1 Jantrawut, Pensak
A1 Rachtanapun, Pornchai
A1 Ruksiriwanich, Warintorn
K1 5α-reductase
K1 RD69
K1 SRD5A2
K1 Tubtim chumphae
K1 androgenetic alopecia
K1 molecular dynamics
K1 rice bran
K1 tocopherol
AB Steroid 5-alpha reductases (SRD5As) are responsible for the conversion of testosterone to dihydrotestosterone, a potent androgen, which is the aetiologic factor of androgenetic alopecia. This study aimed to compare the SRD5A gene expression suppression activity exerted by Thai rice bran extracts and their components and investigate the interactional mechanism between bioactive compounds and SRD5A2 using molecular dynamics (MD) simulation. Bran of Oryza sativa cv. Tubtim Chumphae (TRB), Yamuechaebia Morchor (YRB), Riceberry (RRB), and Malinil Surin (MRB), all rice milling by-products, was solvent-extracted. The ethanolic extract of TRB had the highest sum of overall bioactive compounds (γ-oryzanol; α-, β-, and γ-tocopherol; phenolics; and flavonoids). Among all extracts, TRB greatly downregulated the expression of SRD5A1, SRD5A2, and SRD5A3; there were no significant differences between TRB and finasteride regarding SRD5A suppression. The linear relationship and principal component analysis supported that the α-tocopherol content was correlated with the SRD5A suppression exerted by TRB. Furthermore, MD simulation demonstrated that α-tocopherol had the highest binding affinity towards SRD5A2 by interacting with residues Phe118 and Trp201. Our findings indicate that α-tocopherol effectively downregulates the expression of SRD5A genes and inhibits SRD5A2 activity, actions that are comparable to standard finasteride. TRB, a source of α-tocopherol, could be developed as an anti-hair loss product.
PB MDPI AG
SN 2079-7737
YR 2021
FD 2021-04-09
LK http://hdl.handle.net/10668/17686
UL http://hdl.handle.net/10668/17686
LA en
NO Khantham C, Yooin W, Sringarm K, Sommano SR, Jiranusornkul S, Carmona FD, et al. Effects on Steroid 5-Alpha Reductase Gene Expression of Thai Rice Bran Extracts and Molecular Dynamics Study on SRD5A2. Biology (Basel). 2021 Apr 9;10(4):23.
NO This research project is supported by National Research Council of Thailand (NRCT): NRCT5-RRI63004-P05 and partially supported by Chiang Mai University.
DS RISalud
RD Apr 9, 2025