RT Journal Article T1 Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs A1 Gil-Ocaña, Violeta A1 Jimenez, Isabel M. A1 Mayorga, Cristobalina A1 Doña, Inmaculada A1 Céspedes, Jose Antonio A1 Montañez, Maria I. A1 Vida, Yolanda A1 Torres, Maria J. A1 Perez-Inestrosa, Ezequiel K1 Dendrimeric antigen K1 Silica nanoparticles K1 Biepitope nanocomposites K1 Diagnostic test K1 Drug allergy K1 Penicillins K1 Specific IgE K1 Aprobación de pruebas de diagnóstico K1 Hipersensibilidad a las drogas K1 Penicilinas AB β-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug-protein conjugates that both induce the reactions and are immunologically recognized. Dendrimeric Antigen-Silica particle composites (DeAn@SiO2), consisting on nanoparticles decorated with BL-DeAns are promising candidates for improving the in vitro clinical diagnostic practice. In this nano-inspired system biology, the synthetic dendrimer plays the role of the natural carrier protein, emulating its haptenation by drugs and amplifying the multivalence. Herein, we present the design and synthesis of new multivalent mono- and bi-epitope DeAn@SiO2, using amoxicillin and/or benzylpenicillin allergenic determinants as ligands. The homogeneous composition of nanoparticles provides high reproducibility and quality, which is critical for in vitro applications. The suitable functionalization of nanoparticles allows the anchoring of DeAn, minimizing the nonspecific interactions and facilitating the effective exposure to specific IgE; while the larger interaction area increments the likelihood of capturing specific IgE. This achievement is particularly important for improving sensitivity of current immunoassays since IgE levels in BL allergic patients are very low. Our data suggest that these new nano-based platforms provide a suitable tool for testing IgE recognition to more than one BL simultaneously. Immunochemical studies evidence that mono and bi-epitope DeAn@SiO2 composites could potentially allow the diagnosis of patients allergic to any of these drugs with a single test. These organic-inorganic hybrid materials represent the basis for the development of a single screening for BL-allergies. PB Frontiers YR 2021 FD 2021-12-03 LK http://hdl.handle.net/10668/3456 UL http://hdl.handle.net/10668/3456 LA en NO Gil-Ocaña V, Jimenez IM, Mayorga C, Doña I, Céspedes JA, Montañez MI, et al. Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs. Front Immunol. 2021 Dec 3;12:750109. DS RISalud RD Jul 1, 2025