RT Journal Article
T1 Analysis of Global and Local DNA Methylation Patterns in Blood Samples of Patients With Autism Spectrum Disorder.
A1 García-Ortiz, María Victoria
A1 de la Torre-Aguilar, María José
A1 Morales-Ruiz, Teresa
A1 Gómez-Fernández, Antonio
A1 Flores-Rojas, Katherine
A1 Gil-Campos, Mercedes
A1 Martin-Borreguero, Pilar
A1 Ariza, Rafael R
A1 Roldán-Arjona, Teresa
A1 Perez-Navero, Juan Luis
K1 DNA methylation patterns
K1 NCAM
K1 NGF
K1 autism spectrum disorder
K1 neurodevelopmental regression
AB The goal of this investigation was to determine whether there are alterations in DNA methylation patterns in children with autism spectrum disorder (ASD). Material and Methods: Controlled prospective observational case-control study. Within the ASD group, children were sub-classified based on the presence (AMR subgroup) or absence (ANMR subgroup) of neurodevelopmental regression during the first 2 years of life. We analyzed the global levels of DNA methylation, reflected in LINE-1, and the local DNA methylation pattern in two candidate genes, Neural Cell Adhesion Molecule (NCAM1) and Nerve Growth Factor (NGF) that, according to our previous studies, might be associated to an increased risk for ASD. For this purpose, we utilized blood samples from pediatric patients with ASD (n = 53) and their corresponding controls (n = 45). Results: We observed a slight decrease in methylation levels of LINE-1 in the ASD group, compared to the control group. One of the CpG in LINE-1 (GenBank accession no.X58075, nucleotide position 329) was the main responsible for such reduction, highly significant in the ASD subgroup of children with AMR (p
SN 2296-2360
YR 2021
FD 2021-10-05
LK https://hdl.handle.net/10668/25860
UL https://hdl.handle.net/10668/25860
LA en
DS RISalud
RD Apr 8, 2025