RT Journal Article
T1 Stroma-derived but not tumor ADAMTS1 is a main driver of tumor growth and metastasis
A1 Fernandez-Rodriguez, Ruben
A1 Javier Rodriguez-Baena, Francisco
A1 Martino-Echarri, Estefania
A1 Peris-Torres, Carlos
A1 del Carmen Plaza-Calonge, Maria
A1 Carlos Rodriguez-Manzaneque, Juan
K1 extracellular protease
K1 extracellular matrix
K1 hypoxia
K1 tumor stroma
K1 vasculature
K1 Expression
K1 Gene
K1 Host
K1 Thrombospondin-1
K1 Angiogenesis
K1 Inhibition
K1 Deficiency
K1 Activation
K1 Induction
K1 Proteases
AB The matrix metalloprotease ADAMTS1 (A Disintegrin And Metalloprotease with ThromboSpondin repeats 1) has been involved in tumorigenesis although its contributions appeared ambiguous. To understand the multifaceted actions of this protease, it is still required a deeper knowledge of its implication in heterogeneous tumor-stroma interactions. Using a syngeneic B16F1 melanoma model in wild type and ADAMTS1 knockout mice we found distinct stroma versus tumor functions for this protease. Genetic deletion of ADAMTS1 in the host microenvironment resulted in a drastic decrease of tumor growth and metastasis. However, the downregulation of tumor ADAMTS1 did not uncover relevant effects. Reduced tumors in ADAMTS1 KO mice displayed a paradoxical increase in vascular density and vascular-related genes; a detailed characterization revealed an impaired vasculature, along with a minor infiltration of macrophages. In addition, ex-vivo assays supported a chief role for ADAMTS1 in vascular sprouting, and melanoma xenografts showed a relevant induction of its expression in stroma compartments. These findings provide the first genetic evidence that supports the pro-tumorigenic role of stromal ADAMTS1.
PB Impact journals llc
YR 2016
FD 2016-06-07
LK http://hdl.handle.net/10668/19428
UL http://hdl.handle.net/10668/19428
LA en
DS RISalud
RD Apr 8, 2025