RT Journal Article
T1 Use of Granada medium to detect group B streptococcal colonization in pregnant women.
A1 Rosa-Fraile, Manuel
A1 Rodriguez-Granger, Javier
A1 Cueto-Lopez, Marina
A1 Sampedro, Antonio
A1 Biel Gaye, Enrique
A1 Haro, José Manuel
A1 Andreu, Antonia
K1 Medios de cultivo
K1 Embarazo
K1 Complicaciones infecciosas del embarazo
K1 Estudios prospectivos
K1 Infecciones estreptocócicas
K1 Técnicas de tipificación bacteriana
K1 Granada
K1 España
AB Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comparatively, GM was found to be as sensitive as the selective broth for the detection of GBS in vaginal specimens and more sensitive than selective broth for the detection of GBS in vaginoanorectal samples (96 versus 82%). The use of GM improved the time to reporting of a GBS-positive result by at least 24 h and reduced the direct cost of screening. We have also found that the inconvenience of anaerobic incubation of GM plates can be avoided when a cover slide is placed upon the inoculum, because aerobic incubation in GM plates with cover slides causes GBS to develop the same pigmentation that it develops with incubation under anaerobic conditions. These data support the routine use of GM plates or tubes as a more accurate, easier, and cheaper method of identification of GBS-colonized women compared to the enrichment broth technique.
PB American Society for Microbiology
SN 0095-1137
YR 1999
FD 1999-08
LK http://hdl.handle.net/10668/2123
UL http://hdl.handle.net/10668/2123
LA en
NO Rosa-Fraile M, Rodriguez-Granger J, Cueto-Lopez M, Sampedro A, Biel Gaye E, Haro JM, et al. Use of Granada medium to detect group B streptococcal colonization in pregnant women. J Clin Microbiol. 1999; 37(8):2674-7.
NO Journal Article;
DS RISalud
RD Apr 7, 2025