RT Journal Article
T1 Monocytes Phenotype and Cytokine Production in Human Immunodeficiency Virus-1 Infected Patients Receiving a Modified Vaccinia Ankara-Based HIV-1 Vaccine: Relationship to CD300 Molecules Expression.
A1 Vitalle, Joana
A1 Zenarruzabeitia, Olatz
A1 Terren, Iñigo
A1 Plana, Montserrat
A1 Guardo, Alberto C
A1 Leal, Lorna
A1 Peña, Jose
A1 Garcia, Felipe
A1 Borrego, Francisco
K1 CD300
K1 CD300c
K1 CD300f
K1 HIV-1 vaccine
K1 Human immunodeficiency virus
K1 Lipopolysaccharide
K1 Monocytes
K1 Therapeutic vaccine
AB A modified vaccinia Ankara-based HIV-1 vaccine clade B (MVA-B) has been tested for safety and immunogenicity in low-risk human immunodeficiency virus (HIV)-uninfected individuals and as a therapeutic vaccine in HIV-1-infected individuals on combined antiretroviral therapy (cART). As a therapeutic vaccine, MVA-B was safe and broadly immunogenic; however, patients still showed a viral rebound upon treatment interruption. Monocytes are an important part of the viral reservoir and several studies suggest that they are partly responsible for the chronic inflammation observed in cART-treated HIV-infected people. The CD300 family of receptors has an important role in several diseases, including viral infections. Monocytes express CD300a, c, e, and f molecules and lipopolysaccharide (LPS) and other stimuli regulate their expression. However, the expression and function of CD300 receptors on monocytes in HIV infection is still unknown. In this work, we investigated for the first time the expression of CD300 molecules and the cytokine production in response to LPS on monocytes from HIV-1-infected patients before and after vaccination with MVA-B. Our results showed that CD300 receptors expression on monocytes from HIV-1-infected patients correlates with markers of HIV infection progression and immune inflammation. Specifically, we observed a positive correlation between the expression of CD300e and CD300f receptors on monocytes with the number of CD4+ T cells of HIV-1-infected patients before vaccination. We also saw a positive correlation between the expression of the inhibitory receptor CD300f and the expression of CD163 on monocytes from HIV-1-infected individuals before and after vaccination. In addition, monocytes exhibited a higher cytokine production in response to LPS after vaccination, almost at the same levels of monocytes from healthy donors. Furthermore, we also described a correlation in the expression of CD300e and CD300f receptors with TNF-α production in response to LPS, only in monocytes of HIV-1-infected patients before vaccination. Altogether, our results describe the impact of HIV-1 and of the MVA-B vaccine in cytokine production and monocytes phenotype.
PB Frontiers Research Foundation
SN 1664-3224
YR 2017
FD 2017-07-03
LK http://hdl.handle.net/10668/11486
UL http://hdl.handle.net/10668/11486
LA en
NO Vitallé J, Zenarruzabeitia O, Terrén I, Plana M, Guardo AC, Leal L, et al. Monocytes Phenotype and Cytokine Production in Human Immunodeficiency Virus-1 Infected Patients Receiving a Modified Vaccinia Ankara-Based HIV-1 Vaccine: Relationship to CD300 Molecules Expression. Front Immunol. 2017 Jul 21;8:836
NO The authors thank all of the patients and healthy donors who participated in the study and the staff of the Hospital Clìnic, Barcelona, who cared for the patients. This study was supported by grants from “Plan Estatal de I+ D+ I 2013–2016, ISCIII-Subdirección de Evaluación y Fomento de la Investigación-Fondo Europeo de Desarrollo Regional (FEDER) (Grants PI13/00889, PI15/00480), and Marie Curie Actions, Career Integration Grant, European commission (Grant CIG 631674).” The study was also partially supported by grants: EC10-153, TRA-094, SAF2015-66193-R, RIS [Red Temática Cooperativa de Grupos de Investigación en Sida del Fondo de Investigación Sanitaria (FIS)], HIVACAT (Catalan Program for the development of HIV-1 vaccines). JV is recipient of a predoctoral contract from the Department of Education, Language Policy, and Culture, Basque Government and a fellowship from the Jesús de Gangoiti Barrera Foundation.
DS RISalud
RD Apr 19, 2025