RT Journal Article
T1 3D and organoid culture in research: physiology, hereditary genetic diseases and cancer.
A1 Suarez-Martinez, Elisa
A1 Suazo-Sanchez, Irene
A1 Celis-Romero, Manuel
A1 Carnero, Amancio
K1 3D culturing
K1 Cancer
K1 Disease modeling
K1 Organoid
K1 Stem cells
AB In nature, cells reside in tissues subject to complex cell-cell interactions, signals from extracellular molecules and niche soluble and mechanical signaling. These microenvironment interactions are responsible for cellular phenotypes and functions, especially in normal settings. However, in 2D cultures, where interactions are limited to the horizontal plane, cells are exposed uniformly to factors or drugs; therefore, this model does not reconstitute the interactions of a natural microenvironment. 3D culture systems more closely resemble the architectural and functional properties of in vivo tissues. In these 3D cultures, the cells are exposed to different concentrations of nutrients, growth factors, oxygen or cytotoxic agents depending on their localization and communication. The 3D architecture also differentially alters the physiological, biochemical, and biomechanical properties that can affect cell growth, cell survival, differentiation and morphogenesis, cell migration and EMT properties, mechanical responses and therapy resistance. This latter point may, in part, explain the failure of current therapies and affect drug discovery research. Organoids are a promising 3D culture system between 2D cultures and in vivo models that allow the manipulation of signaling pathways and genome editing of cells in a body-like environment but lack the many disadvantages of a living system. In this review, we will focus on the role of stem cells in the establishment of organoids and the possible therapeutic applications of this model, especially in the field of cancer research.
PB BioMed Central Ltd.
SN 2045-3701
YR 2022
FD 2022-04-01
LK http://hdl.handle.net/10668/20368
UL http://hdl.handle.net/10668/20368
LA en
NO Suarez-Martinez E, Suazo-Sanchez I, Celis-Romero M, Carnero A. 3D and organoid culture in research: physiology, hereditary genetic diseases and cancer. Cell Biosci. 2022 Apr 1;12(1):39.
DS RISalud
RD Apr 10, 2025