RT Journal Article
T1 Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital.
A1 Villalón, Pilar
A1 Valdezate, Sylvia
A1 Cabezas, Teresa
A1 Ortega, Montserrat
A1 Garrido, Noelia
A1 Vindel, Ana
A1 Medina-Pascual, María J
A1 Saez-Nieto, Juan A
K1 A baumannii
K1 PFGE
K1 sequence type
K1 Acinetobacter baumannii
K1 MLVA
K1 Genotipo
K1 Clone
K1 ADN Bacteriano
K1 Tipificación de Secuencias Multilocus
AB BACKGROUNDNosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period.RESULTSPFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively.CONCLUSIONSPFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.
PB BioMed Central
YR 2015
FD 2015-02-25
LK http://hdl.handle.net/10668/2281
UL http://hdl.handle.net/10668/2281
LA en
NO Villalón P, Valdezate S, Cabezas T, Ortega M, Garrido N, Vindel A, et al. Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital. BMC Microbiol. 2015; 15:47
NO Journal Article;
DS RISalud
RD Apr 10, 2025