RT Journal Article
T1 Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain.
A1 Dashti, Alejandro
A1 Santin, Monica
A1 Köster, Pamela C
A1 Bailo, Begoña
A1 Ortega, Sheila
A1 Imaña, Elena
A1 Habela, Miguel Angel
A1 Rivero-Juarez, Antonio
A1 Vicente, Joaquin
A1 Arnal, Maria C
A1 de Luco, Daniel Fernandez
A1 Morrondo, Patrocinio
A1 Armenteros, Jose A
A1 Balseiro, Ana
A1 Cardona, Guillermo A
A1 Martinez-Carrasco, Carlos
A1 Ortiz, Jose Antonio
A1 Calero-Bernal, Rafael
A1 Carmena, David
A1 Gonzalez-Barrio, David
K1 Enterocytozoon bieneusi
K1 Spain
K1 Molecular diversity
K1 Wild ungulates
AB Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals.
PB Oxford University Press
YR 2022
FD 2022-09-08
LK http://hdl.handle.net/10668/19811
UL http://hdl.handle.net/10668/19811
LA en
NO Dashti A, Santín M, Köster PC, Bailo B, Ortega S, Imaña E, et al. Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain. Med Mycol. 2022 Sep 30;60(9):myac070
NO Sampling in the Basque Country was conducted by members of the Association for the Defence of the Game Natural Heritage of the Basque Country (ARTIO). We thank the Dirección General del Medio Natural y Planificación Rural del Principado de Asturias (Oviedo, Spain). Antonio RiveroJuárez is the recipient of a Miguel Servet Research Contract by the Ministerio de Ciencia, Promoción y Universidades of Spain (CP18/00111). D.G.B. is the recipient of a Sara Borrell Research Contract (CD19CIII/00011) funded by the Spanish Ministry of Science, Innovation and Universities. A.D. is the recipient of a PFIS contract (FI20CIII/00002) funded by the Spanish Ministry of Science and Innovation and Universities.
DS RISalud
RD Apr 8, 2025