RT Journal Article
T1 Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs.
A1 Vaz-Rodrigues, Rita
A1 Ferreras-Colino, Elisa
A1 Ugarte-Ruiz, Maria
A1 Pesciaroli, Michele
A1 Thomas, Jobin
A1 Garcia-Seco, Teresa
A1 Sevilla, Iker A
A1 Perez-Sancho, Marta
A1 Mateo, Rafael
A1 Dominguez, Lucas
A1 Gortazar, Christian
A1 Risalde, Maria A
K1 Heat-inactivated Mycobacterium bovis
K1 Salmonella Choleraesuis
K1 Pig
K1 Trained immunity
AB Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still facing problems regarding the control of distinct serovars. Therefore, we hypothesized that an immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have an immune training effect in pigs challenged with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) and decided to explore the amplitude of this non-specific immune response. For this purpose, twenty-four 10 days-old female piglets were randomly separated in three groups: immunized group (n = 10) received orally two doses of HIMB prior to the intratracheal S. Choleraesuis-challenge, positive control group (n = 9) that was only challenged with S. Choleraesuis, and negative control group (n = 5) that was neither immunized nor infected. All individuals were necropsied 21 days post-challenge. HIMB improved weight gain and reduced respiratory symptoms and pulmonary lesions caused by S. Choleraesuis in pigs. Pigs immunized with HIMB showed higher cytokine production, especially of serum TNFα and lung CCL28, an important mediator of mucosal trained immunity. Moreover, immunized pigs showed lower levels of the biomarker of lipid oxidation malondialdehyde and higher activity of the antioxidant enzyme superoxide dismutase than untreated challenged pigs. However, the excretion and tissue colonization of S. Choleraesuis remained unaffected. This proof-of-concept study suggests beneficial clinical, pathological, and heterologous immunological effects against bacterial pathogens within the concept of trained immunity, opening avenues for further research.
PB BioMed Central
YR 2022
FD 2022-03-17
LK http://hdl.handle.net/10668/20366
UL http://hdl.handle.net/10668/20366
LA en
NO Vaz-Rodrigues R, Ferreras-Colino E, Ugarte-Ruíz M, Pesciaroli M, Thomas J, García-Seco T, et al. Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs. Vet Res. 2022 Apr 18;53(1):31
DS RISalud
RD Apr 8, 2025