RT Journal Article
T1 SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination.
A1 Silva, Sonia
A1 Altmannova, Veronika
A1 Eckert-Boulet, Nadine
A1 Kolesar, Peter
A1 Gallina, Irene
A1 Hang, Lisa
A1 Chung, Inn
A1 Arneric, Milica
A1 Zhao, Xiaolan
A1 Buron, Line Due
A1 Mortensen, Uffe H
A1 Krejci, Lumir
A1 Lisby, Michael
K1 Homologous recombination
K1 Rad51
K1 Rad52
K1 Rad59
K1 SUMOylation
K1 Srs2
AB Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR.
YR 2016
FD 2016-04-16
LK http://hdl.handle.net/10668/10041
UL http://hdl.handle.net/10668/10041
LA en
DS RISalud
RD Apr 8, 2025