RT Journal Article
T1 Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain?
A1 García, B E
A1 Martínez-Aranguren, R
A1 Bernard Alonso, A
A1 Gamboa, P
A1 Feo Brito, F
A1 Bartra, J
A1 Blanca-López, N
A1 Gómez, F
A1 Alvarado, M I
A1 Fernández, J
A1 Alonso, M D
A1 Gonzalez-Mancebo, E
A1 Moya, C
A1 Parra, A
A1 Terrados, S
A1 Sola, L
A1 Goikoetxea, M J
A1 Sanz, M L
K1 Microarray
K1 Pollen allergy
K1 Molecular diagnosis
K1 Accuracy
K1 Expresión Génica
K1 Humanos
K1 Inmunoglobulina E
K1 Masculino
K1 Análisis por Micromatrices
K1 Mediana Edad
K1 Polen
K1 Valor Predictivo de las Pruebas
K1 Profilinas
K1 Curva ROC
K1 Hipersensibilidad Respiratoria
K1 España
K1 Especificidad de la Especie
K1 Árboles
K1 Alérgenos
K1 Área Bajo la Curva
K1 Adulto
K1 Femenino
AB BACKGROUNDMultiple sensitization is frequent among pollen-allergic patients. The goal of this study was to determine the diagnostic accuracy of the ImmunoCAP ISAC 112 (ISAC112) microarray in allergy to pollen from several taxa and its clinical utility in a Spanish population.METHODSSpecific IgE was determined in 390 pollen-allergic patients using the ISAC112 microarray. Diagnostic accuracy (sensitivity, specificity, predictive values, and area under the ROC curve) was calculated for the diagnosis of allergy to pollen from grass (n=49), cypress (n=75), olive tree (n=33), plane tree (n=63), and pellitory of the wall (n=17) and compared with that of the singleplex ImmunoCAP immunoassay.RESULTSThe sensitivity of the ISAC112 microarray ranged from 68.2% for allergy to plane tree pollen to 93.9% for allergy to grass pollen. The specificity was >90%. The AUC for the diagnosis of allergy to plane tree pollen was 0.798, whereas the AUC for the remaining cases was ≥0.876. The accuracy of ISAC112 was higher than that of ImmunoCAP for plane tree pollen and similar for the remaining pollens. The frequency of sensitization to most species-specific allergenic components and profilins varied between the different geographical regions studied. A total of 73% of pollen-allergic patients were sensitized to species-specific components of more than 1 pollen type.CONCLUSIONSThe ISAC112 microarray is an accurate tool for the diagnosis of allergy to pollen from grass, cypress, olive tree, plane tree, and pellitory of the wall. The features of the ISAC112 microarray are similar or superior (in the case of plane tree pollen) to those of ImmunoCAP. This microarray is particularly useful for the etiologic diagnosis of pollinosis in patients sensitized to multiple pollen species whose pollination periods overlap.
PB Esmon Publicidad
SN 1018-9068
YR 2016
FD 2016
LK http://hdl.handle.net/10668/2503
UL http://hdl.handle.net/10668/2503
LA en
NO García BE, Martínez-Aranguren R, Bernard Alonso A, Gamboa P, Feo Brito F, Bartra J, et al. Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain? J Investig Allergol Clin Immunol. 2016; 26(2):92-9
NO Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 20, 2025