RT Journal Article
T1 Substrate adhesion determines migration during mesenchymal cell condensation in
A1 Casanellas, Ignasi
A1 Jiang, Hongkai
A1 David, Carolyn M.
A1 Vida, Yolanda
A1 Perez-Inestrosa, Ezequiel
A1 Samitier, Josep
A1 Lagunas, Anna
K1 Cell migration
K1 Mesenchymal condensation
K1 Nanopatterned substrates
K1 Arginine-glycine-aspartic acid
K1 RGD
K1 Chondrogenesis
K1 N-cadherin
K1 Fibronectin
K1 Dynamics
K1 Expression
K1 Model
K1 Chondrogenesis
K1 Alpha-v-beta-3
K1 Gastrulation
K1 Involvement
K1 Mechanisms
AB Mesenchymal condensation is a prevalent morphogenetic transition that is essential in chondrogenesis. However, the current understanding of condensation mechanisms is limited. In vivo, progenitor cells directionally migrate from the surrounding loose mesenchyme towards regions of increasing matrix adherence (the condensation centers), which is accompanied by the upregulation of fibronectin. Here, we focused on the mechanisms of cell migration during mesenchymal cell condensation and the effects of matrix adherence. Dendrimer-based nanopatterns of the cell-adhesive peptide arginine-glycine-aspartic acid (RGD), which is present in fibronectin, were used to regulate substrate adhesion. We recorded collective and single-cell migration of mesenchymal stem cells, under chondrogenic induction, using live-cell imaging. Our results show that the cell migration mode of single cells depends on substrate adhesiveness, and that cell directionality controls cell condensation and the fusion of condensates. Inhibition experiments revealed that cell???cell interactions mediated by N-cadherin (also known as CDH2) are also pivotal for directional migration of cell condensates by maintaining cell???cell cohesion, thus suggesting a fine interplay between cell???matrix and cell???cell adhesions. Our results shed light on the role of cell interactions with a fibronectin-depositing matrix during chondrogenesis in vitro, with possible applications in regenerative medicine.
PB Company biologists ltd
SN 0021-9533
YR 2022
FD 2022-11-01
LK http://hdl.handle.net/10668/20410
UL http://hdl.handle.net/10668/20410
LA en
DS RISalud
RD Apr 12, 2025