RT Journal Article
T1 Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta.
A1 Rueda-Martínez, Carmen
A1 Lamas, Óscar
A1 Mataró, María José
A1 Robledo-Carmona, Juan
A1 Sánchez-Espín, Gemma
A1 Jiménez-Navarro, Manuel
A1 Such-Martínez, Miguel
A1 Fernández, Borja
K1 Enfermedades de las Válvulas Cardíacas
K1 Válvula Aórtica
K1 Algoritmos
K1 Aneurisma de la Aorta
K1 Reacción en Cadena en Tiempo Real de la Polimerasa
K1 Demografía
K1 Expresión Génica
K1 Dilatación Patológica
K1 Reacción en Cadena de la Polimerasa
K1 ADN Complementario
K1 Desnaturalización de Ácido Nucleico
K1 Humanos
AB Dilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms leading to AAD associated with BAV are still poorly understood. The search for differentially expressed genes in diseased tissue by quantitative real-time PCR (qPCR) is an invaluable tool to fill this gap. However, studies dedicated to identify reference genes necessary for normalization of mRNA expression in aortic tissue are scarce. In this report, we evaluate the qPCR expression of six candidate reference genes in tissue from the ascending aorta of 52 patients with a variety of clinical and demographic characteristics, normal and dilated aortas, and different morphologies of the aortic valve (normal aorta and normal valve n = 30; dilated aorta and normal valve n = 10; normal aorta and BAV n = 4; dilated aorta and BAV n = 8). The expression stability of the candidate reference genes was determined with three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable genes for the three algorithms employed were CDKN1β, POLR2A and CASC3, independently of the structure of the aorta and the valve morphology. In conclusion, we propose the use of these three genes as reference genes for mRNA expression analysis in human ascending aorta. However, we suggest searching for specific reference genes when conducting qPCR experiments with new cohort of samples.
PB Public Library of Science
YR 2014
FD 2014-05-19
LK http://hdl.handle.net/10668/1639
UL http://hdl.handle.net/10668/1639
LA en
NO Rueda-Martínez C, Lamas O, Mataró MJ, Robledo-Carmona J, Sánchez-Espín G, Jiménez-Navarro M, et al. Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta. PLoS ONE. 2014; 9(5):e97449
NO Journal Article;
DS RISalud
RD Apr 12, 2025