RT Journal Article
T1 Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions.
A1 Solana, Carmen
A1 Ferrer-Luque, Carmen M
A1 Wang, Xu
A1 Baca, Pilar
A1 Ruiz-Linares, Matilde
A1 Cabrera, Teresa
K1 NaOCl
K1 alkaline EDTANa4
K1 cetrimide
K1 cytotoxicity
K1 irrigating solutions
AB The aim of this study is to determine the cytotoxic effects of tetrasodium ethylenediaminetetraacetic acid (EDTANa4) when used alone or when combined with sodium hypochlorite (NaOCl), with and without the addition of cetrimide (CTR). Human pulmonary fibroblast cell line was exposed to the following irrigating solutions: group 1, 2.5% NaOCl; group 2, 10% EDTANa4; group 3, 20% EDTANa4; group 4, 2.5% NaOCl/5% EDTANa4; group 5, 2.5% NaOCl/10% EDTANa4; group 6, 2.5% NaOCl/5% EDTANa4/0.2% CTR; group 7, 2.5% NaOCl/10% EDTANa4/0.2% CTR; group 8, control, cells in Dulbecco's modified Eagle's medium. Methyl thiazol tetrazolium assay was used to determine the viability of cells after 1 and 24 h. Viability percentages were analyzed for global comparison using the Welch test followed by the Games-Howell test to determine groups with similar viability, and the Student's t test was used to compare the two times. The lowest viability was obtained with a 2.5% NaOCl solution at both time periods. The association of NaOCl with EDTANa4 increased the cellular viability in direct relation with the concentration of the chelating agent. Globally, after 24 h of exposure, cell viability reduced. The solutions of EDTANa4 showed moderate cytotoxic effects when compared with NaOCl alone.
YR 2020
FD 2020-05-31
LK http://hdl.handle.net/10668/15664
UL http://hdl.handle.net/10668/15664
LA en
DS RISalud
RD Apr 9, 2025