RT Journal Article
T1 A comprehensive study of calcific aortic stenosis: from rabbit to human samples.
A1 Mourino-Alvarez, Laura
A1 Baldan-Martin, Montserrat
A1 Sastre-Oliva, Tamara
A1 Martin-Lorenzo, Marta
A1 Maroto, Aroa Sanz
A1 Corbacho-Alonso, Nerea
A1 Rincon, Raul
A1 Martin-Rojas, Tatiana
A1 Lopez-Almodovar, Luis Fernando
A1 Alvarez-Llamas, Gloria
A1 Vivanco, Fernando
A1 Padial, Luis Rodriguez
A1 de la Cuesta, Fernando
A1 Barderas, Maria Gonzalez
K1 Aortic stenosis
K1 Cardiovascular
K1 Proteomics
K1 Rabbit model
AB The global incidence of calcific aortic stenosis (CAS) is increasing owing, in part, to a growing elderly population. The condition poses a great challenge to public health, because of the multiple comorbidities of these older patients. Using a rabbit model of CAS, we sought to characterize protein alterations associated with calcified valve tissue that can be ultimately measured in plasma as non-invasive biomarkers of CAS. Aortic valves from healthy and mild stenotic rabbits were analyzed by two-dimensional difference gel electrophoresis, and selected reaction monitoring was used to directly measure the differentially expressed proteins in plasma from the same rabbits to corroborate their potential as diagnostic indicators. Similar analyses were performed in plasma from human subjects, to examine the suitability of these diagnostic indicators for transfer to the clinical setting. Eight proteins were found to be differentially expressed in CAS tissue, but only three were also altered in plasma samples from rabbits and humans: transitional endoplasmic reticulum ATPase, tropomyosin α-1 chain and L-lactate dehydrogenase B chain. Results of receiver operating characteristic curves showed the discriminative power of the scores, which increased when the three proteins were analyzed as a panel. Our study shows that a molecular panel comprising three proteins related to osteoblastic differentiation could have utility as a serum CAS indicator and/or therapeutic target.
YR 2018
FD 2018-06-19
LK https://hdl.handle.net/10668/25568
UL https://hdl.handle.net/10668/25568
LA en
DS RISalud
RD Apr 11, 2025