RT Journal Article
T1 RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR.
A1 Gentile, Adriana-Mariel
A1 Lhamyani, Said
A1 Coín-Aragüez, Leticia
A1 Oliva-Olivera, Wilfredo
A1 Zayed, Hatem
A1 Vega-Rioja, Antonio
A1 Monteseirin, Javier
A1 Romero-Zerbo, Silvana-Yanina
A1 Tinahones, Francisco J
A1 Bermúdez-Silva, Francisco-Javier
A1 El Bekay, Rajaa
K1 Adipocitos
K1 Adipogénesis
K1 Procesos biológicos
K1 Índice de masa corporal
K1 Expresión génica
K1 Humanos
K1 Resistencia a la insulina
K1 Reacción en cadena de la polimerasa en tiempo real
K1 Células del estroma
K1 Grasa subcutánea
AB Real-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.
PB Public Libray of Science
YR 2016
FD 2016-06-15
LK http://hdl.handle.net/10668/2443
UL http://hdl.handle.net/10668/2443
LA en
NO Gentile AM, Lhamyani S, Coín-Aragüez L, Oliva-Olivera W, Zayed H, Vega-Rioja A, et al. RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR. PLoS ONE. 2016; 11(6):e0157002
NO Journal Article;
DS RISalud
RD Apr 6, 2025