RT Journal Article
T1 GPX3 Overexpression in Cumulus Cells Entails a Poor Prognosis for Uterine Implantation of Morphotype A Embryos.
A1 Bejarano, Ignacio
A1 Dorado-Silva, Mónica
A1 Sarmiento-Soto, Helia
A1 Álvarez-Sánchez, Nuria
A1 Lardone, Patricia Judith
A1 Guerrero, Juan Miguel
A1 Sánchez-Martín, Pascual
A1 Carrillo-Vico, Antonio
K1 GPX3
K1 cumulus cells
K1 implantation
K1 infertility
K1 oocyte competence
K1 transcriptome
AB Morphological embryo quality is an accurate prognostic tool for the success of assisted reproduction implantation, although complete certainty cannot be guaranteed. The transcriptome of the cumulus cells could be monitored as a faithful reflex of the physiological state of the oocytes, given the molecular crosstalk between both types of cells. Here, we compare the expression of specific genes related to oocyte competence, such as hyaluronic acid synthase 2 (HAS2), cell division control protein 42 (CDC42), connexin 43 (CX43), and glutathione peroxidase 3 (GPX3), in cumulus cells from implanted versus non-implanted embryos in 25 women, using RT-qPCR. After embryo transfer, two cohorts were differentiated: the pregnant group (women with the implantation of 100% of embryos transferred) versus the non-pregnant group (with an absence of embryo implantation), aiming to compare the possible differential expression of the selected genes in the cumulus cells of embryos from each group. HAS2, CDC42 and CX43 did not reveal differential expression between the two cohorts. However, GPX3 showed significantly reduced expression in the cumulus belonging to the pregnant group. Interestingly, even cumulus cells belonging only to morphotype A embryos showed a significantly lower expression of GPX3 in the pregnancy group. GPX3 overexpression in cumulus cells could be a poor prognostic indicator of implantation, discriminating beyond the capacity of the morphokinetic score. Unveiling the cumulus transcriptome could improve successful implantation in assisted reproduction treatments.
SN 2079-7737
YR 2022
FD 2022-09-16
LK http://hdl.handle.net/10668/20803
UL http://hdl.handle.net/10668/20803
LA en
DS RISalud
RD Apr 8, 2025