RT Journal Article
T1 Atherosclerotic Pre-Conditioning Affects the Paracrine Role of Circulating Angiogenic Cells Ex-Vivo
A1 Eslava-Alcon, Sara
A1 Extremera-García, M. Jesús
A1 Sanchez-Gomar, Ismael
A1 Beltrán-Camacho, Lucía
A1 Rosal-Vela, Antonio
A1 Muñoz, Javier
A1 Ibarz, Nuria
A1 Alonso-Piñero, Jose Angel
A1 Rojas-Torres, Marta
A1 Jiménez-Palomares, Margarita
A1 González-Rovira, Almudena
A1 Conejero, Rosario
A1 Doiz, Esther
A1 Rodriguez-Piñero, Manuel
A1 Moreno-Luna, Rafael
A1 Durán-Ruiz, M. Carmen
K1 Circulating angiogenic cells
K1 Endothelial colony forming cells
K1 Paracrine role
K1 Secretome
K1 Atherosclerotic factors
K1 Angiogenesis
K1 Proteomics
K1 Células
K1 Secretoma
K1 Aterosclerosis
K1 Inhibidores de la angiogénesis
K1 Proteómica
K1 Células progenitoras endoteliales
AB In atherosclerosis, circulating angiogenic cells (CAC), also known as early endothelial progenitor cells (eEPC), are thought to participate mainly in a paracrine fashion by promoting the recruitment of other cell populations such as late EPC, or endothelial colony-forming cells (ECFC), to the injured areas. There, ECFC replace the damaged endothelium, promoting neovascularization. However, despite their regenerative role, the number and function of EPC are severely affected under pathological conditions, being essential to further understand how these cells react to such environments in order to implement their use in regenerative cell therapies. Herein, we evaluated the effect of direct incubation ex vivo of healthy CAC with the secretome of atherosclerotic arteries. By using a quantitative proteomics approach, 194 altered proteins were identified in the secretome of pre-conditioned CAC, many of them related to inhibition of angiogenesis (e.g., endostatin, thrombospondin-1, fibulins) and cell migration. Functional assays corroborated that healthy CAC released factors enhanced ECFC angiogenesis, but, after atherosclerotic pre-conditioning, the secretome of pre-stimulated CAC negatively affected ECFC migration, as well as their ability to form tubules on a basement membrane matrix assay. Overall, we have shown here, for the first time, the effect of atherosclerotic factors over the paracrine role of CAC ex vivo. The increased release of angiogenic inhibitors by CAC in response to atherosclerotic factors induced an angiogenic switch, by blocking ECFC ability to form tubules in response to pre-conditioned CAC. Thus, we confirmed here that the angiogenic role of CAC is highly affected by the atherosclerotic environment.
PB MDPI
YR 2020
FD 2020-07-24
LK http://hdl.handle.net/10668/3799
UL http://hdl.handle.net/10668/3799
LA en
NO Eslava-Alcon S, Extremera-García MJ, Sanchez-Gomar I, Beltrán-Camacho L, Rosal-Vela A, Muñoz J, et al. Atherosclerotic Pre-Conditioning Affects the Paracrine Role of Circulating Angiogenic Cells Ex-Vivo. Int J Mol Sci. 2020 Jul 24;21(15):5256
DS RISalud
RD Apr 30, 2025