RT Journal Article
T1 The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4.
A1 Begley, Victoria
A1 Corzo, Daniel
A1 Jordán-Pla, Antonio
A1 Cuevas-Bermúdez, Abel
A1 Miguel-Jiménez, Lola de
A1 Pérez-Aguado, David
A1 Machuca-Ostos, Mercedes
A1 Navarro, Francisco
A1 Chávez, María José
A1 Pérez-Ortín, José E
A1 Chávez, Sebastián
AB Co-transcriptional imprinting of mRNA by Rpb4 and Rpb7 subunits of RNA polymerase II (RNAPII) and by the Ccr4-Not complex conditions its post-transcriptional fate. In turn, mRNA degradation factors like Xrn1 are able to influence RNAPII-dependent transcription, making a feedback loop that contributes to mRNA homeostasis. In this work, we have used repressible yeast GAL genes to perform accurate measurements of transcription and mRNA degradation in a set of mutants. This genetic analysis uncovered a link from mRNA decay to transcription elongation. We combined this experimental approach with computational multi-agent modelling and tested different possibilities of Xrn1 and Ccr4 action in gene transcription. This double strategy brought us to conclude that both Xrn1-decaysome and Ccr4-Not regulate RNAPII elongation, and that they do it in parallel. We validated this conclusion measuring TFIIS genome-wide recruitment to elongating RNAPII. We found that xrn1Δ and ccr4Δ exhibited very different patterns of TFIIS versus RNAPII occupancy, which confirmed their distinct role in controlling transcription elongation. We also found that the relative influence of Xrn1 and Ccr4 is different in the genes encoding ribosomal proteins as compared to the rest of the genome.
YR 2019
FD 2019
LK http://hdl.handle.net/10668/14377
UL http://hdl.handle.net/10668/14377
LA en
DS RISalud
RD Apr 13, 2025