RT Journal Article
T1 Fluorescent BAPAD Dendrimeric Antigens Are Efficiently Internalized by Human Dendritic Cells
A1 Mesa-Antunez, Pablo
A1 Collado, Daniel
A1 Vida, Yolanda
A1 Najera, Francisco
A1 Fernandez, Tahia
A1 Torres, Maria Jose
A1 Perez-Inestrosa, Ezequiel
K1 Dendrimeric antigen
K1 Naphthalimide
K1 Fluorescence
K1 Flow cytometry
K1 Confocal microscopy
K1 Amoxicillin
K1 Allergy
AB A new fluorescent dendrimeric antigen (DeAn) based on a dendron with amoxicilloyl terminal groups was synthesized. The synthesis was carried out using a novel class of all-aliphatic polyamide dendrimer (BisAminoalkylPolyAmide Dendrimers, or BAPAD) involving the direct condensation of 3,3'-diazidopivalic acid as a building block. Iterative azide reduction/amide formation increases the dendrimer generation. The BAPAD dendrimer was designed with a cystamine core. Reduction of the disulfide bond allows the incorporation of BAPAD dendrons into a 1,8-naphthalimide functionalized with a maleimide group. The fluorescence properties of DeAn were studied in PBS and compared with the properties of an equivalent dendron possessing amino-terminal groups. Both molecules shown high fluorescence quantum yields in PBS and could readily be visualized by fluorescence microscopy. DeAn was used as a synthetic antigen in a biomedical assay that tests their potential as an amoxicillin carrier in drug internalization by dendritic cells (DC) from tolerant and allergic patients. Cytometry data suggest that the dendrons are non-toxic and easily internalized by DCs, while confocal microscopy images indicate that the compounds are preferentially accumulated in the cytoplasm. These results indicate that BAPAD dendrons are good candidates for synthetic scaffolds for biomedical applications.
PB MDPI
SN 2073-4360
YR 2016
FD 2016-04-01
LK http://hdl.handle.net/10668/19320
UL http://hdl.handle.net/10668/19320
LA en
NO Mesa-Antunez P, Collado D, Vida Y, Najera F, Fernandez T, Torres MJ, Perez-Inestrosa E. Fluorescent BAPAD Dendrimeric Antigens Are Efficiently Internalized by Human Dendritic Cells. Polymers (Basel). 2016 Mar 26;8(4):111
NO This research was supported by several sources: Ministerio de Ciencia e Innovación-Spain (CTQ2013-41339-P and CTQ2015-71896-REDT), Junta de Andalucía-Spain (PI-0159/2013). RIRAAF (RD12/0013/0003 and 0001), and Instituto de Salud Carlos III (PS12/02529). This research was co-financed by FEDER funds. We gratefully acknowledge the computer resources, technical expertise, and assistance provided by the SCBI (Supercomputing and bioinformatics) center of the University of Malaga. Pablo Mesa-Antunez acknowledges the FPI fellowship he was granted (CTQ2010-20303).
DS RISalud
RD Apr 8, 2025