RT Journal Article
T1 Rapid diagnosis of human brucellosis by peripheral-blood PCR assay.
A1 Queipo-Ortuño, María Isabel
A1 Morata, Pilar
A1 Ocón, Pilar
A1 Manchado, Pedro
A1 Colmenero, Juan de Dios
K1 Anticuerpos bacterianos
K1 brucelosis
K1 Cebadores de ADN
K1 ADN bacteriano
K1 Fiebre
K1 Reacción en cadena de la polimerasa
K1 Valores de referencia
K1 Reproducibilidad de los resultados
K1 Sensibilidad y especificidad
AB A single-step PCR assay with genus-specific primers for the amplification of a 223-bp region of the sequence encoding a 31-kDa immunogenetic Brucella abortus protein (BCSP31) was used for the rapid diagnosis of human brucellosis. We examined peripheral blood from 47 patients, with a total of 50 cases of brucellosis, and a group of 60 control subjects, composed of patients with febrile syndromes of several etiologies other than brucellosis, asymptomatic subjects seropositive for Brucella antibodies, and healthy subjects. Diagnosis of brucellosis was established in 35 cases (70%) by isolation of Brucella in blood culture and in the other 15 cases (30%) by clinical and serological means. The sensitivity of our PCR assay was 100%, since it correctly identified all 50 cases of brucellosis, regardless of the duration of the disease, the positivity of the blood culture, or the presence of focal forms. The specificity of the test was 98.3%, and the only false-positive result was for a patient who had had brucellosis 2 months before and possibly had a self-limited relapse. In those patients who relapsed, the results of our PCR assay were positive for both the initial infection and the relapse, becoming negative once the relapse treatment was completed and remaining negative in the follow-up tests at 2, 4, and 6 months. In conclusion, these results suggest that the PCR assay is rapid and easy to perform and highly sensitive and specific, and it may therefore be considered a useful tool for diagnosis of human brucellosis.
PB American Society for Microbiology
SN 0095-1137
YR 1997
FD 1997-11
LK http://hdl.handle.net/10668/2142
UL http://hdl.handle.net/10668/2142
LA en
NO Queipo-Ortuño MI, Morata P, Ocón P, Manchado P, Colmenero JD. Rapid diagnosis of human brucellosis by peripheral-blood PCR assay. J Clin Microbiol. 1997; 35(11):2927-30
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025