RT Journal Article
T1 ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans.
A1 Kieffer, Nicolas
A1 Guzman-Puche, Julia
A1 Poirel, Laurent
A1 Kang, Hyo Jung
A1 Jeon, Che Ok
A1 Nordmann, Patrice
AB Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment. The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme. The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime. This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs.
PB Oxford University Press
YR 2019
FD 2019-01-16
LK http://hdl.handle.net/10668/13586
UL http://hdl.handle.net/10668/13586
LA en
NO Kieffer N, Guzmán-Puche J, Poirel L, Kang HJ, Jeon CO, Nordmann P. ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans. J Antimicrob Chemother. 2019 Jun 1;74(6):1568-1571
DS RISalud
RD Apr 8, 2025