%0 Journal Article
%A Kieffer, Nicolas
%A Guzman-Puche, Julia
%A Poirel, Laurent
%A Kang, Hyo Jung
%A Jeon, Che Ok
%A Nordmann, Patrice
%T ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans.
%D 2019
%U http://hdl.handle.net/10668/13586
%X Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment. The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme. The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime. This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs.
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