RT Journal Article
T1 Cytotoxicity and Effects on the Synapsis Induced by Pure Cylindrospermopsin in an E17 Embryonic Murine Primary Neuronal Culture in a Concentration- and Time-Dependent Manner.
A1 Hinojosa, María G
A1 Prieto, Ana I
A1 Muñoz-Castro, Clara
A1 Sánchez-Mico, María V
A1 Vitorica, Javier
A1 Cameán, Ana M
A1 Jos, Ángeles
K1 cylindrospermopsin
K1 cytotoxicity
K1 neurotoxicity
K1 primary neuronal cultures
K1 synapsis
AB Cylindrospermopsin (CYN) is a cyanotoxin whose incidence has been increasing in the last decades. Due to its capacity to exert damage at different levels of the organism, it is considered a cytotoxin. Although the main target organ is the liver, recent studies indicate that CYN has potential toxic effects on the nervous system, both in vitro and in vivo. Thus, the aim of the present work was to study the effects of this cyanotoxin on neuronal viability and synaptic integrity in murine primary cultures of neurons exposed to environmentally relevant concentrations (0-1 µg/mL CYN) for 12, 24, and 48 h. The results demonstrate a concentration- and time-dependent decrease in cell viability; no cytotoxicity was detected after exposure to the cyanotoxin for 12 h, while all of the concentrations assayed decreased this parameter after 48 h. Furthermore, CYN was also demonstrated to exert damage at the synaptic level in a murine primary neuronal culture in a concentration- and time-dependent manner. These data highlight the importance of studying the neurotoxic properties of this cyanotoxin in different experimental models.
YR 2022
FD 2022-02-26
LK http://hdl.handle.net/10668/21592
UL http://hdl.handle.net/10668/21592
LA en
DS RISalud
RD Apr 4, 2025