RT Journal Article
T1 Inflammatory response in human lung cells stimulated with plasma from COPD patients.
A1 Arellano-Orden, Elena
A1 Calero-Acuña, Carmen
A1 Sánchez-López, Verónica
A1 Carrasco-Hernández, Laura
A1 Márquez-Martín, Eduardo
A1 Ortega-Ruiz, Francisco
A1 Otero-Candelera, Remedios
A1 Marín-Hinojosa, Carmen
A1 López-Campos, José Luis
K1 COPD
K1 CRP
K1 IL-8
K1 MMP-9
K1 inflammation
AB Chronic obstructive pulmonary disease (COPD) is a condition resulting from a persistent inflammatory state in the airways even after smoking cessation. Intriguingly, the reasons behind this persistence of the inflammatory influx without smoking exposure have not been fully unraveled. We aimed to explore the hypothesis that systemic inflammation in COPD patients influences lung cell inflammatory response. We cultured human lung fibroblast and human airway epithelial cell lines with plasma from COPD patients (four emphysematous-COPD, four asthma-COPD overlap, four chronic bronchitis-COPD, and four bronchiectasis- COPD), and four smokers or ex-smokers without COPD as controls. Non-stimulated cells were used as controls. We measured Interleukine-8 (IL-8), C-reactive protein (CRP) and matrix metalloproteinase-9 (MMP-9) in plasma and culture supernatants by ELISA. Cells stimulated with plasma from COPD patients and non-COPD smoker subjects produced higher CRP, IL- 8 and MMP-9 levels, an increase for COPD in CRP (p=0.029) in epithelial cells and IL-8 (p=0.039) in fibroblasts and decrease for MMP-9 (p=0.039) in fibroblasts, compared with non-stimulated cells. The response was higher in epithelial cells for IL-8 (p=0.003) and in fibroblasts for MMP-9 (p=0.063). The plasma from chronic bronchitis and bronchiectasis phenotypes induced higher IL-8 in fibroblasts. Plasma from COPD patients increases the inflammatory response in lung epithelial cells and lung fibroblasts, with a different response depending on the cell type and clinical phenotype.
SN 1828-695X
YR 2022
FD 2022-05-24
LK http://hdl.handle.net/10668/21621
UL http://hdl.handle.net/10668/21621
LA en
DS RISalud
RD Apr 6, 2025