RT Journal Article
T1 Novel Molecular Characterization of Colorectal Primary Tumors Based on miRNAs.
A1 Moreno, Elisa Conde
A1 Pascual, Alejandro
A1 Prieto-Cuadra, Daniel
A1 Laza, Val F
A1 Molina-Cerrillo, Javier
A1 Ramos-Muñoz, Miren Edurne
A1 Rodríguez-Serrano, Esperanza Macarena
A1 Soto, José Luis
A1 Carrato, Alfredo
A1 García-Bermejo, María Laura
A1 Guillén-Ponce, Carmen
K1 biomarkers
K1 colorectal cancer
K1 miRNAs
K1 patient stratification
AB microRNAs (miRNA) expression in colorectal (CR) primary tumours can facilitate a more precise molecular characterization. We identified and validated a miRNA profile associated with clinical and histopathological features that might be useful for patient stratification. In situ hybridization array using paraffin-embedded biopsies of CR primary tumours were used to screen 1436 miRNAs. 17 miRNAs were selected for validation by quantitative reverse transcription polymerase chain reaction (qRT-PCR) (n = 192) and were further correlated with clinical and histopathological data. We demonstrated that miRNAs associated to Colorectal Cancer (CRC) diagnosis age (over 50s and 60s) included miR-1-3p, miR-23b-3p, miR-27b-3p, miR-143-3p, miR-145-5p and miR-193b-5p. miR-23b-3p and miR-24-3p discriminated between Lynch Syndrome and sporadic CRC. miR-10a-5p, miR-20a-5p, miR-642b and Let-7a-5p were associated to stroma abundance. miR-642b and Let-7a-5p were associated with to peritumoral inflammation abundance. miR-1-3p, miR-143-3p and miR-145-5p correlated with mucinous component. miR-326 correlated with tumour location (right or left sided). miR-1-3p associated with tumour grade. miR-20a-5p, miR-193b-5p, miR-320a, miR-326 and miR-642b-3p associated to tumour stage and progression. Remarkably, we also demonstrated that miR-1-3p and miR-326 expression significantly associated with patient overall survival (OS). Hierarchical clustering and bioinformatics analysis indicated that selected miRNAs could re-classify the patients and work cooperatively, modulating common target genes involved in colorectal cancer key signalling pathways. In conclusion, molecular characterization of CR primary tumours based on miRNAs could lead to more accurate patient reclassification and may be useful for efficient patient management.
SN 2072-6694
YR 2019
FD 2019-03-11
LK https://hdl.handle.net/10668/27055
UL https://hdl.handle.net/10668/27055
LA en
DS RISalud
RD Apr 8, 2025