RT Journal Article
T1 Primary Cilia in Chondrogenic Differentiation of Equine Bone Marrow Mesenchymal Stem Cells: Ultrastructural Study
A1 Jose Luesma, Maria
A1 Cantarero, Irene
A1 Ranera, Beatriz
A1 Rosa Remacha, Ana
A1 Castiella, Tomas
A1 Romero, Antonio
A1 Martin, Inmaculada
A1 Rodellar, Clementina
A1 Junquera, Concepcion
K1 Primary cilium chondrocyte
K1 Chondrocyte ultrastructure
K1 Equine BM-MSCs
AB Locomotor system disorders in equine species, such as tendon lesions, osteoarthritis, or ligament injuries, are some of the most frequent causes of dramatic reduction in horse performance. Traditional therapies are aimed at the inflammatory process and pain, but they do not regenerate normal tendon or ligament matrix and do not reduce re-injured rates. Mesenchymal stem cells have started to use as therapeutic option to repair these injured tissues. Most studies have focused on their isolation, in vitro culture and pheno-typing. However, mesenchymal stem cell ultrastructure has been disregarded in the last years. We investigate the ultrastructural characteristics of these cells once differentiated into chondrocytes. Ultrastructural analysis was conducted on suspension cultures of differentiated chondrocytes from bone marrow mesenchymal stem cells by means of transmission electron microscopy. The morphologic characteristics of these cells, their ability to produce the extracellular matrix, and the presence of a single cilium could be indicative of the mesenchymal cells differentiation into chondrocyte phenotype. This study provides essential data to evaluate the degree of suitable phenotypic stability, for these cells can be used with repair purposes. 
PB Elsevier
SN 0737-0806
YR 2016
FD 2016-12-01
LK http://hdl.handle.net/10668/18814
UL http://hdl.handle.net/10668/18814
LA en
NO Luesma MJ, Cantarero I, Ranera B, Remacha AR, Castiella T, Romero A, et al. Primary Cilia in Chondrogenic Differentiation of Equine Bone Marrow Mesenchymal Stem Cells: Ultrastructural Study. Journal Of Equine Veterinary Science [Internet]. 1 de diciembre de 2016;47:47-54.
DS RISalud
RD Apr 5, 2025