RT Journal Article
T1 Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype
A1 Jesus, Hendor N. R.
A1 Rocha, Danilo J. P. G.
A1 Ramos, Rommel T. J.
A1 Silva, Artur
A1 Brenig, Bertram
A1 Goes-Neto, Aristoteles
A1 Costa, Mateus M.
A1 Soares, Siomar C.
A1 Azevedo, Vasco
A1 Aguiar, Eric R. G. R.
A1 Martinez-Martinez, Luiz
A1 Ocampo, Alain
A1 Alibi, Sana
A1 Dorta, Alexis
A1 Pacheco, Luis G. C.
A1 Navas, Jesus
K1 Corynebacterium amycolatum
K1 Pan-genome
K1 Multidrug resistance
K1 Emerging pathogen
K1 Virulence factor
K1 Mycolic acids
K1 Cell-wall
K1 Identification
K1 Iron
K1 Siderophore
K1 Diphtheriae
K1 Pilus
K1 Virulence
K1 Islands
K1 System
AB Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the MDR invasive phenotype in clinical isolates. In this study, we performed a comprehensive pan-genomic analysis of C. amycolatum, including 26 isolates from different countries. We obtained the novel genome sequences of 8 of them, which are multidrug resistant clinical isolates from Spain and Tunisia. They were analyzed together with other 18 complete or draft C. amycolatum genomes retrieved from GenBank. The species C. amycolatum presented an open pan-genome (alpha = 0.854905), with 3,280 gene families, being 1,690 (51.52%) in the core genome, 1,121 related to accessory genes (34.17%), and 469 related to unique genes (14.29%). Although some classic corynebacterial virulence factors are absent in the species C. amycolatum, we did identify genes associated with immune evasion, toxin, and antiphagocytosis among the predicted putative virulence factors. Additionally, we found genomic evidence for extensive acquisition of antimicrobial resistance genes through genomic islands.
PB Frontiers Research Foundation
YR 2022
FD 2022-10-17
LK http://hdl.handle.net/10668/20629
UL http://hdl.handle.net/10668/20629
LA en
NO Jesus HNR, Rocha DJPG, Ramos RTJ, Silva A, Brenig B, Góes-Neto A, et al. Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype. Front Microbiol. 2022 Nov 16;13:1011578
DS RISalud
RD Apr 14, 2025