RT Journal Article
T1 IL8 and EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality.
A1 Kofanova, Olga
A1 Bellora, Camille
A1 Quesada, Rocio Aguilar
A1 Bulla, Alexandre
A1 Panadero-Fajardo, Sonia
A1 Keipes, Marc
A1 Shea, Kathi
A1 Stone, Mars
A1 Lescuyer, Pierre
A1 Betsou, Fay
K1 PBMC
K1 Preanalytical
K1 Quality control
AB Uncontrolled preanalytical variables can reduce the accuracy and reproducibility of downstream analytical results from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from EDTA and citrate-anticoagulated blood samples, obtained from healthy subjects and patients with inflammatory and infectious conditions. PBMC-derived RNA samples were examined for gene expression changes induced by extended blood pre-centrifugation delays at 4 °C and RT. We used Taqman RTqPCR to evaluate the combination of two target genes for their "diagnostic performance" in identifying EDTA and citrate-anticoagulated PBMC samples with extended pre-centrifugation times. We established the PBMC preanalytical score, a gene expression metric to asses the PBMC quality related to the pre-centrifugation delay at room temperature for different anticoagulants. The PBMC preanalytical score measurement can identify: CONCLUSION: The proposed PBMC preanalytical score may enable objective PBMC sample qualification for downstream applications, which may be influenced by blood precentrifugation delays.
YR 2018
FD 2018-11-26
LK http://hdl.handle.net/10668/13258
UL http://hdl.handle.net/10668/13258
LA en
DS RISalud
RD Apr 9, 2025