%0 Journal Article
%A Kofanova, Olga
%A Bellora, Camille
%A Quesada, Rocio Aguilar
%A Bulla, Alexandre
%A Panadero-Fajardo, Sonia
%A Keipes, Marc
%A Shea, Kathi
%A Stone, Mars
%A Lescuyer, Pierre
%A Betsou, Fay
%T IL8 and EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality.
%D 2018
%U http://hdl.handle.net/10668/13258
%X Uncontrolled preanalytical variables can reduce the accuracy and reproducibility of downstream analytical results from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from EDTA and citrate-anticoagulated blood samples, obtained from healthy subjects and patients with inflammatory and infectious conditions. PBMC-derived RNA samples were examined for gene expression changes induced by extended blood pre-centrifugation delays at 4 °C and RT. We used Taqman RTqPCR to evaluate the combination of two target genes for their "diagnostic performance" in identifying EDTA and citrate-anticoagulated PBMC samples with extended pre-centrifugation times. We established the PBMC preanalytical score, a gene expression metric to asses the PBMC quality related to the pre-centrifugation delay at room temperature for different anticoagulants. The PBMC preanalytical score measurement can identify: CONCLUSION: The proposed PBMC preanalytical score may enable objective PBMC sample qualification for downstream applications, which may be influenced by blood precentrifugation delays.
%K PBMC
%K Preanalytical
%K Quality control
%~