RT Journal Article
T1 MicroRNA-200c Attenuates the Tumor-Infiltrating Capacity of Macrophages.
A1 Raue, Rebecca
A1 Frank, Ann-Christin
A1 Fuhrmann, Dominik C
A1 de la Cruz-Ojeda, Patricia
A1 Rösser, Silvia
A1 Bauer, Rebekka
A1 Cardamone, Giulia
A1 Weigert, Andreas
A1 Syed, Shahzad Nawaz
A1 Schmid, Tobias
A1 Brüne, Bernhard
K1 breast tumor
K1 macrophage
K1 miR
K1 tumor microenvironment
AB Macrophages constitute a major part of the tumor-infiltrating immune cells. Within the tumor microenvironment, they acquire an alternatively activated, tumor-supporting phenotype. Factors released by tumor cells are crucial for the recruitment of tumor-associated macrophages. In the present project, we aimed to understand the role of hsa-miR-200c-3p (miR-200c) in the interplay between tumor cells and macrophages. To this end, we employed a coculture system of MCF7 breast tumor cells and primary human macrophages and observed the transfer of miR-200c from apoptotic tumor cells to macrophages, which required intact CD36 receptor in macrophages. We further comprehensively determined miR-200c targets in macrophages by mRNA-sequencing and identified numerous migration-associated mRNAs to be downregulated by miR-200c. Consequently, miR-200c attenuated macrophage infiltration into 3-dimensional tumor spheroids. miR-200c-mediated reduction in infiltration further correlated with a miR-200c migration signature comprised of the four miR-200c-repressed, predicted targets PPM1F, RAB11FIB2, RDX, and MSN.
SN 2079-7737
YR 2022
FD 2022-02-22
LK http://hdl.handle.net/10668/20800
UL http://hdl.handle.net/10668/20800
LA en
DS RISalud
RD Apr 18, 2025