RT Journal Article
T1 Comparison between urine culture profile and morphology classification using fluorescence parameters of the Sysmex UF-1000i urine flow cytometer.
A1 Jiménez-Guerra, G
A1 Heras-Cañas, V
A1 Valera-Arcas, M D
A1 Rodríguez-Grangér, J
A1 Navarro, J M
A1 Gutiérrez-Fernández, J
K1 diagnosis
K1 fluorescence
K1 urinary tract infection
K1 urine culture
K1 urine flow cytometer
AB To determine the usefulness of the fluorescence parameters generated by Sysmex UF-1000i flow cytometer for the rapid diagnosis of urinary tract infection by bacilli or cocci. Urine samples (n = 1924) were studied by culture and microbiology and subsequently by cytometry, using BACT-Morph software and considering forward-scattered light (FSC) and fluorescent light scatter fluorescence parameters. BACT-Morph software showed moderate diagnostic accuracy (78·4%) to detect rod-shaped bacteria, with sensitivity of 82·4% and specificity of 62·5%. Forward-scattered (B_FSC) values of the bacterial channel were significant higher for the Gram-positive cocci category (P  Utilization of BACT-Morph software and bacterial channel fluorescence parameters (B_FSC ≥24·2 ch) offered an approximate discrimination of bacilli and cocci but the specificity was low, especially for FSC. Further research is needed to establish the usefulness of flow cytometry for aetiological diagnosis.
YR 2017
FD 2017-01-04
LK http://hdl.handle.net/10668/10614
UL http://hdl.handle.net/10668/10614
LA en
DS RISalud
RD Apr 5, 2025