RT Journal Article
T1 Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration.
A1 Reyes-Peces, Maria V
A1 Felix, Eduardo
A1 Martinez-Vazquez, Francisco J
A1 Fernandez-Montesinos, Rafael
A1 Bomati-Miguel, Oscar
A1 Mesa-Diaz, Maria Del Mar
A1 Alcantara, Rodrigo
A1 Vilches-Perez, Jose Ignacio
A1 Salido, Mercedes
A1 De la Rosa-Fox, Nicolas
A1 Piñero, Manuel
K1 3D scaffold
K1 Bone tissue engineering
K1 Cytoskeleton
K1 Focal adhesion
K1 Hybrid
K1 Laser micromachining
K1 Osteoblasts
K1 Regenerative medicine
K1 Robocasting
K1 Sol-gel ink
AB The design and synthesis of sol-gel silica-based hybrid materials and composites offer significant benefits to obtain innovative biomaterials with controlled porosity at the nanostructure level for applications in bone tissue engineering. In this work, the combination of robocasting with sol-gel ink of suitable viscosity prepared by mixing tetraethoxysilane (TEOS), gelatin and β-tricalcium phosphate (β-TCP) allowed for the manufacture of 3D scaffolds consisting of a 3D square mesh of interpenetrating rods, with macropore size of 354.0 ± 17.0 μm, without the use of chemical additives at room temperature. The silica/gelatin/β-TCP system underwent irreversible gelation, and the resulting gels were also used to fabricate different 3D structures by means of an alternative scaffolding method, involving high-resolution laser micromachining by laser ablation. By this way, 3D scaffolds made of 2 mm thick rectangular prisms presenting a parallel macropore system drilled through the whole thickness and consisting of laser micromachined holes of 350.8 ± 16.6-micrometer diameter, whose centers were spaced 1312.0 ± 23.0 μm, were created. Both sol-gel based 3D scaffold configurations combined compressive strength in the range of 2-3 MPa and the biocompatibility of the hybrid material. In addition, the observed Si, Ca and P biodegradation provided a suitable microenvironment with significant focal adhesion development, maturation and also enhanced in vitro cell growth. In conclusion, this work successfully confirmed the feasibility of both strategies for the fabrication of new sol-gel-based hybrid scaffolds with osteoconductive properties.
PB MDPI
YR 2022
FD 2022-10-07
LK http://hdl.handle.net/10668/20976
UL http://hdl.handle.net/10668/20976
LA en
NO Reyes-Peces MV, Félix E, Martínez-Vázquez FJ, Fernández-Montesinos R, Bomati-Miguel Ó, Mesa-Díaz MDM, Alcántara R, et al. Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration. Gels. 2022 Oct 7;8(10):634
NO Authors acknowledge the use of instrumentation as well as the technical advice provided by the GEMA-Uex research group from Universidad de Extremadura (UNEX) with robocasting equipment, as well as SCCYT (UCA) for SEM, ICP and EA divisions as well as SCBM at the University of Cadiz. The authors would also like to thank. J. Vilches-Troya, retired Professorof Histology and Pathology of the University of Cadiz, for his expert advice and supervision, and Enrique Gallero-Rebollo for his assistance in figure design. All individuals included in this section have consented the acknowledgment
DS RISalud
RD Apr 7, 2025