RT Journal Article
T1 Metabolic changes of Interleukin-12/15/18-stimulated human NK cells.
A1 Terrén, Iñigo
A1 Orrantia, Ane
A1 Mosteiro, Alba
A1 Vitallé, Joana
A1 Zenarruzabeitia, Olatz
A1 Borrego, Francisco
AB Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their persistence in the host and their increased effector functions. Several studies have shown that NK cells modulate their metabolism in response to cytokine-stimulation and other stimuli, suggesting that there is a link between metabolism and cellular functions. In this paper, we have analyzed metabolic changes associated to IL-12/15/18-stimulation and the relevance of glycolytic pathway for NK cell effector functions. We have found CIML NK cells are able to retain a metabolic profile shifted towards glycolysis seven days after cytokine withdrawal. Furthermore, we found that treatment with 2-DG differently affects distinct NK cell effector functions and is stimuli-dependent. These findings may have implications in the design of NK cell-based cancer immunotherapies.
YR 2021
FD 2021-03-19
LK https://hdl.handle.net/10668/27653
UL https://hdl.handle.net/10668/27653
LA en
DS RISalud
RD Apr 5, 2025