RT Journal Article
T1 The Janus Role of Adhesion in Chondrogenesis
A1 Casanellas, Ignasi
A1 Lagunas, Anna
A1 Vida, Yolanda
A1 Pérez-Inestrosa, Ezequiel
A1 Andrades, José A.
A1 Becerra, José
A1 Samitier, Josep
K1 Dendrimer
K1 Nanopatterning
K1 RGD
K1 Mesenchymal cell condensation
K1 Cell–cell interactions
K1 YAP
K1 Chondrogenesis
K1 Dendrímeros
K1 Comunicación celular
K1 Condrogénesis
AB Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
PB MDPI
YR 2020
FD 2020-07-24
LK http://hdl.handle.net/10668/3581
UL http://hdl.handle.net/10668/3581
LA en
NO Casanellas I, Lagunas A, Vida Y, Pérez-Inestrosa E, Andrades JA, Becerra J, Samitier J. The Janus Role of Adhesion in Chondrogenesis. Int J Mol Sci. 2020 Jul 24;21(15):5269.
DS RISalud
RD Apr 20, 2025