RT Journal Article
T1 LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma
A1 Arenas, Alberto M.
A1 Cuadros, Marta
A1 Andrades, Alvaro
A1 García, Daniel J.
A1 Coira, Isabel F.
A1 Rodríguez, María Isabel
A1 Baliñas-Gavira, Carlos
A1 Peinado, Paola
A1 Álvarez-Pérez, Juan Carlos
A1 Medina, Pedro P.
K1 Adenocarcinoma of lung
K1 Biomarkers
K1 Tumor
K1 RNA
K1 Long noncoding
K1 Adenocarcinoma del pulmón
K1 Biomarcadores
K1 Neoplasias
K1 ARN
K1 ARN largo no codificante
AB Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing roles in different pathologies including cancer. Over the last years, growing scientific evidence supports the key role of some lncRNAs in tumor development and proposes them as valuable biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA, DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines. We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity (AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential lung cancer biomarker.
PB MDPI
YR 2020
FD 2020-07-28
LK http://hdl.handle.net/10668/3508
UL http://hdl.handle.net/10668/3508
LA en
NO Arenas AM, Cuadros M, Andrades A, García DJ, Coira IF, Rodríguez MI, et al. LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma. Cancers. 2020 Jul 28;12(8):2080
DS RISalud
RD Apr 8, 2025