RT Journal Article
T1 Olfactory Neuroepithelium Cells from Cannabis Users Display Alterations to the Cytoskeleton and to Markers of Adhesion, Proliferation and Apoptosis.
A1 Delgado-Sequera, Alejandra
A1 Hidalgo-Figueroa, Maria
A1 Barrera-Conde, Marta
A1 Duran-Ruiz, Mª Carmen
A1 Castro, Carmen
A1 Fernandez-Aviles, Cristina
A1 de la Torre, Rafael
A1 Sanchez-Gomar, Ismael
A1 Perez, Victor
A1 Geribaldi-Doldan, Noelia
A1 Robledo, Patricia
A1 Berrocoso, Esther
K1 Adhesion
K1 Apoptosis
K1 Cannabis
K1 Cytoskeleton
K1 Human olfactory neuroepithelium
K1 Proteomic
AB Cannabis is the third most commonly used psychoactive substance of abuse, yet it also receives considerable attention as a potential therapeutic drug. Therefore, it is essential to fully understand the actions of cannabis in the human brain. The olfactory neuroepithelium (ON) is a peripheral nervous tissue that represents an interesting surrogate model to study the effects of drugs in the brain, since it is closely related to the central nervous system, and sensory olfactory neurons are continually regenerated from populations of stem/progenitor cells that undergo neurogenesis throughout life. In this study, we used ON cells from chronic cannabis users and healthy control subjects to assess alterations in relevant cellular processes, and to identify changes in functional proteomic pathways due to cannabis consumption. The ON cells from cannabis users exhibited alterations in the expression of proteins that were related to the cytoskeleton, cell proliferation and cell death, as well as, changes in proteins implicated in cancer, gastrointestinal and neurodevelopmental pathologies. Subsequent studies showed cannabis provoked an increase in cell size and morphological alterations evident through β-Tubulin III staining, as well as, enhanced beta-actin expression and a decrease in the ability of ON cells to undergo cell attachment, suggesting abnormalities of the cytoskeleton and cell adhesion system. Furthermore, these cells proliferated more and underwent less cell death. Our results indicate that cannabis may alter key processes of the developing brain, some of which are similar to those reported in mental disorders like DiGeorge syndrome, schizophrenia and bipolar disorder.
PB Springer
YR 2020
FD 2020-11-25
LK http://hdl.handle.net/10668/16664
UL http://hdl.handle.net/10668/16664
LA en
NO Delgado-Sequera A, Hidalgo-Figueroa M, Barrera-Conde M, Duran-Ruiz MC, Castro C, Fernández-Avilés C, et al. Olfactory Neuroepithelium Cells from Cannabis Users Display Alterations to the Cytoskeleton and to Markers of Adhesion, Proliferation and Apoptosis. Mol Neurobiol. 2021 Apr;58(4):1695-1710
DS RISalud
RD Mar 14, 2025